GlutaredoxinV1, Main, Exploration, bibRecord, 000933

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast.

Identifieur interne : 000933 ( Main/Exploration ); précédent : 000932; suivant : 000934

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast.

Auteurs : Kyoung-Dong Kim [Corée du Sud] ; Hyo-Jin Kim ; Kyung-Chang Lee ; Jung-Hye Roe

Source :

Biochemical and biophysical research communications [ 1090-2104 ] ; 2011.

RBID : pubmed:21531205

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Motifs (MeSH), GATA Transcription Factors (genetics), GATA Transcription Factors (metabolism), Glutaredoxins (genetics), Glutaredoxins (metabolism), Homeostasis (MeSH), Iron (metabolism), Protein Structure, Tertiary (MeSH), Schizosaccharomyces (genetics), Schizosaccharomyces (metabolism), Schizosaccharomyces pombe Proteins (genetics), Schizosaccharomyces pombe Proteins (metabolism).
MESH :
- chemical , genetics : GATA Transcription Factors, Glutaredoxins, Schizosaccharomyces pombe Proteins.
- chemical , metabolism : GATA Transcription Factors, Glutaredoxins, Iron, Schizosaccharomyces pombe Proteins.
- genetics : Schizosaccharomyces.
- metabolism : Schizosaccharomyces.
- Amino Acid Motifs, Homeostasis, Protein Structure, Tertiary.

Abstract

The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins, Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and iron-utilizing genes regardless of iron availability. In order to unravel how Grx4 is involved in Fep1-mediated regulation, interaction between them was investigated. Co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) revealed that Grx4 physically interacts with Fep1 in vivo. BiFC revealed localized nuclear dots produced by interaction of Grx4 with Fep1. Mutation of cysteine-172 in the CGFS motif to serine (C172S) produced effects similarly observed under Grx4 depletion, such as the loss of iron-dependent gene regulation and the absence of nuclear dots in BiFC analysis. These results suggest that the ability of Grx4 to bind iron, most likely Fe-S cofactor, could be critical in interacting with and modulating the activity of Fep1.

DOI: 10.1016/j.bbrc.2011.04.069
PubMed: 21531205

Affiliations:

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Le document en format XML

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<author><name sortKey="Kim, Kyoung Dong" sort="Kim, Kyoung Dong" uniqKey="Kim K" first="Kyoung-Dong" last="Kim">Kyoung-Dong Kim</name>
<affiliation wicri:level="4"><nlm:affiliation>Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul, Republic of Korea.</nlm:affiliation>
<country xml:lang="fr">Corée du Sud</country>
<wicri:regionArea>Laboratory of Molecular Microbiology, School of Biological Sciences and Institute of Microbiology, Seoul National University, Seoul</wicri:regionArea>
<placeName><settlement type="city">Séoul</settlement>
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<author><name sortKey="Kim, Hyo Jin" sort="Kim, Hyo Jin" uniqKey="Kim H" first="Hyo-Jin" last="Kim">Hyo-Jin Kim</name>
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<author><name sortKey="Roe, Jung Hye" sort="Roe, Jung Hye" uniqKey="Roe J" first="Jung-Hye" last="Roe">Jung-Hye Roe</name>
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<term>Glutaredoxins (genetics)</term>
<term>Glutaredoxins (metabolism)</term>
<term>Homeostasis (MeSH)</term>
<term>Iron (metabolism)</term>
<term>Protein Structure, Tertiary (MeSH)</term>
<term>Schizosaccharomyces (genetics)</term>
<term>Schizosaccharomyces (metabolism)</term>
<term>Schizosaccharomyces pombe Proteins (genetics)</term>
<term>Schizosaccharomyces pombe Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs de transcription GATA (génétique)</term>
<term>Facteurs de transcription GATA (métabolisme)</term>
<term>Fer (métabolisme)</term>
<term>Glutarédoxines (génétique)</term>
<term>Glutarédoxines (métabolisme)</term>
<term>Homéostasie (MeSH)</term>
<term>Motifs d'acides aminés (MeSH)</term>
<term>Protéines de Schizosaccharomyces pombe (génétique)</term>
<term>Protéines de Schizosaccharomyces pombe (métabolisme)</term>
<term>Schizosaccharomyces (génétique)</term>
<term>Schizosaccharomyces (métabolisme)</term>
<term>Structure tertiaire des protéines (MeSH)</term>
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<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>GATA Transcription Factors</term>
<term>Glutaredoxins</term>
<term>Schizosaccharomyces pombe Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>GATA Transcription Factors</term>
<term>Glutaredoxins</term>
<term>Iron</term>
<term>Schizosaccharomyces pombe Proteins</term>
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<term>Protéines de Schizosaccharomyces pombe</term>
<term>Schizosaccharomyces</term>
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<term>Fer</term>
<term>Glutarédoxines</term>
<term>Protéines de Schizosaccharomyces pombe</term>
<term>Schizosaccharomyces</term>
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<term>Homeostasis</term>
<term>Protein Structure, Tertiary</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Homéostasie</term>
<term>Motifs d'acides aminés</term>
<term>Structure tertiaire des protéines</term>
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<front><div type="abstract" xml:lang="en">The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins, Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and iron-utilizing genes regardless of iron availability. In order to unravel how Grx4 is involved in Fep1-mediated regulation, interaction between them was investigated. Co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) revealed that Grx4 physically interacts with Fep1 in vivo. BiFC revealed localized nuclear dots produced by interaction of Grx4 with Fep1. Mutation of cysteine-172 in the CGFS motif to serine (C172S) produced effects similarly observed under Grx4 depletion, such as the loss of iron-dependent gene regulation and the absence of nuclear dots in BiFC analysis. These results suggest that the ability of Grx4 to bind iron, most likely Fe-S cofactor, could be critical in interacting with and modulating the activity of Fep1.</div>
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<Abstract><AbstractText>The fission yeast Schizosaccharomyces pombe contains two CGFS-type monothiol glutaredoxins, Grx4 and Grx5, which are localized primarily in the nucleus and mitochondria, respectively. We observed involvement of Grx4 in regulating iron-responsive gene expression, which is modulated by a repressor Fep1. Lack of Grx4 caused defects not only in growth but also in the expression of both iron-uptake and iron-utilizing genes regardless of iron availability. In order to unravel how Grx4 is involved in Fep1-mediated regulation, interaction between them was investigated. Co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) revealed that Grx4 physically interacts with Fep1 in vivo. BiFC revealed localized nuclear dots produced by interaction of Grx4 with Fep1. Mutation of cysteine-172 in the CGFS motif to serine (C172S) produced effects similarly observed under Grx4 depletion, such as the loss of iron-dependent gene regulation and the absence of nuclear dots in BiFC analysis. These results suggest that the ability of Grx4 to bind iron, most likely Fe-S cofactor, could be critical in interacting with and modulating the activity of Fep1.</AbstractText>
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	Serveur d'exploration sur la glutarédoxine
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Serveur d'exploration sur la glutarédoxine

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast.

Multi-domain CGFS-type glutaredoxin Grx4 regulates iron homeostasis via direct interaction with a repressor Fep1 in fission yeast.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki